《植物生理学报》 2013, 49(12): 1363-1367
通信作者:刘燕;E-mail: chbly@sohu.com;Tel: 010-62336062
摘 要:
本文研究了春石斛‘森禾2006’的拟原球茎(PLBs)诱导、增殖、分化及壮苗和生根, 建立了其组培快繁体系。结果表明: ‘森禾2006’ PLBs最适诱导培养基为1/2MS+TDZ 0.5 mg·L-1+水解酪蛋白2.0 g·L-1; PLBs增殖培养基为1/2MS+水解酪蛋白2.0 g·L-1; PLBs分化培养基为1/2MS+KT 1.0 mg·L-1+NAA 0.1 mg·L-1; 壮苗生根培养基为MS+IBA 0.5 mg·L-1+NAA 0.1 mg·L-1+ 香蕉泥100.0 g·L-1。关键词:春石斛; 拟原球茎(PLBs); 组培快繁; 种苗
收稿:2013-08-06 修定:2013-09-18
资助:国家“十二五”支撑计划课题(2011BAD12B02-01)
Corresponding author: LIU Yan; E-mail: chbly@sohu.com; Tel: 010-62336062
Abstract:
To achieve the mass-scale multiplication of Dendrobium nobile ‘Senhe 2006’, the optimal media for the induction, multiplication and differentiation of protocorm-like bodies (PLBs), strengthening and rooting were studied and the rapid propagation system by tissue culture was established. The results showed that 1/2MS+TDZ 0.5 mg·L-1+casein hydrolysate 2.0 g·L-1 was optimal for PLB induction. 1/2MS+casein hydrolysate 2.0 g·L-1 was the most suitable for PLB multiplication. 1/2MS+KT 1.0 mg·L-1+NAA 0.1 mg·L-1 was the most efficient for PLB differentiation. The optimum medium for strengthening and rooting was MS+IBA 0.5 mg·L-1 +NAA 0.1 mg·L-1+banana homogenate 100.0 g·L-1.Key words: Dendrobium nobile; protocorm-like bodies (PLBs); tissue culture and rapid propagation; plantlets
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